Table 1. KRAS and BRAF mutations identified in individuals with hepatic cavernous hemangiomas.

Patient	Pathological feature/size/location	Age (yr)	Sex	Next generation sequencing (MAF%)	BNA clamp	Sanger sequencing
1	Cavernous hemangioma (6.5 cm in greatest dimension), right lobe	62	Female	K-RAS p.G12D (0.75%)	Mutant	ND
2	Hemangioma of the liver (7.0 × 7.0 × 3.5 cm), left lateral lobe	37	Male	K-RAS p.G12S (0.1%)a; K-RAS p.G12D (0.1%)	Mutant	K-RAS p.G12S
3	Cavernous hemangioma (7 cm in greatest dimension), right lobe	40	Female	K-RAS p.G12S (0.2%)	Mutant	K-RAS p.G12S
4	Two hemangiomas (1.9 and 1.1 cm), right lobe	57	Male	K-RAS p.G13D (0.4%) K-RAS p.G13S (0.47%)	Mutant	ND
5	Cavernous hemangioma with scattered areas of sclerosis (6.3 cm), left lobe	87	Male	K-RAS p.G12D (0.68%)	Mutant	K-RAS p.G12C; K-RAS p.G12S
6	Cavernous hemangioma (10 cm)	48	Female	K-RAS p.G12S (0.31%)	Mutant	ND
7	Cavernous hemangioma with foci of necrosis and fibrosis (8.5 × 6 × 2 cm)	43	Female	K-RAS p.G12S (0.3%) BRAF p.V600M (0.17%)b	Mutant	K-RAS p.G12S; K-RAS p.G12D
8	Cavernous hemangioma of liver with extensive fibrosis, thrombi, and infarct (17.5 × 9 × 9 cm)	48	Male	K-RAS p.G12S (0.35%); K-RAS p.G12D (0.57%) K-RAS p.G13S (0.5%); K-RAS p.G13D (0.43%)	Mutant	K-RAS p.G12S; K-RAS p.G12V
9	Cavernous hemangioma (1.3 cm)	50	Female	K-RAS p.G12S (0.32%)	Mutant	ND
10	Cavernous hemangioma with focal degenerative change, segments 2 and 3	40	Female	K-RAS p.G12S(0.25); K-RAS p.G13S (0.32%)	Mutant	ND
11	Cavernous hemangioma partially sclerosed (19.0 cm)	42	Male	K-RAS p.G13D (0.18%)	Mutant	K-RAS p.G12R
12	Cavernous hemangioma (1.5 cm), right lobe	46	Female	BRAF p.V600M (0.15%)	Mutant	BRAF p.V600M
13	Hemangioma (6.1 cm), left lobe	55	Female	BRAF p.V600M (0.2%)	Mutant	ND
14	Hepatic hemangioma (2.5 cm)	42	Female	BRAF p.V600M (0.18%)	Mutant	ND

MAF, mutant allele frequency.

^aManually verified in NextGENe alignment viewer.

^bSanger sequence not determined.