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. 2020 Jul 1;76(Pt 7):309–313. doi: 10.1107/S2053230X20008584

Table 2. Crystallization.

Method Sitting-drop vapor diffusion
Plate type Intelli-Plate (Art Robbins Instruments)
Temperature (K) 293
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 50 mM Tris–HCl pH 8.0, 5% glycerol
Composition of reservoir solution 30%(w/v) PEG 8000, 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulfate
Volume and ratio of drop 5 µl protein solution and 5 µl reservoir solution (1:1)
Volume of reservoir (µl) 50