Table 2. Crystallization.
| Method | Sitting-drop vapor diffusion |
| Plate type | Intelli-Plate (Art Robbins Instruments) |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 50 mM Tris–HCl pH 8.0, 5% glycerol |
| Composition of reservoir solution | 30%(w/v) PEG 8000, 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulfate |
| Volume and ratio of drop | 5 µl protein solution and 5 µl reservoir solution (1:1) |
| Volume of reservoir (µl) | 50 |