Skip to main content
. 2020 Jul 1;76(Pt 7):302–308. doi: 10.1107/S2053230X20008201

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type VDX
Temperature (K) 294
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 4.7 mM CoA, 15.8 mM MgCl2, 15.8 mM ADP, 10 mM Tris–HCl pH 8.0, 10 mM β-ME
Composition of reservoir solution 17.5%(w/v) polyethylene glycol 3350, 180 mM ammonium tartrate pH 7.0, 100 mM MES pH 6.4
Volume and ratio of drop 1 µl, 1:1
Volume of reservoir (ml) 0.5