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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Circ Res. 2019 Sep 9;125(9):834–846. doi: 10.1161/CIRCRESAHA.119.314794

Figure 1. Cardiac specific Lmna shRNA induces DCM in mice.

Figure 1.

(a) Experimental timeline showing timepoints of virus injection and echocardiogram. Cardiac performance was assessed by echocardiogram at 5.5 week old. H&E and Masson Trichrome (MT) staining was performed on paraffin heart sections taken 4 weeks after control shRNA and Lmna shRNA transduction. Quantification of myocardial fibrosis of MT sections is shown, virus dose, 2.0E+13 vg/kg, n ≥ 5. Scale bars: 1000 μm for complete heart images; 100 μm for enlarged images. (b) Paraffin heart section immunostained for Lamin A/C (red), PCM1 (green) and DAPI (blue) in mice transduced with control shRNA and Lmna shRNA, scale bar = 100 μm. (c, d) Western blot and quantitative analysis of Lamin A/C protein levels in isolated cardiomyocytes (c) and phospho-Smad2 protein levels in mouse heart tissues (d), n ≥ 3. Data were normalized to Lamin B1. (e-g) Paraffin heart sections (left) and quantifications (right) of (e) αSMA (red), (f) Iba-1 (red) and (g) CD3 (red), cTnI (green) and DAPI (blue) positive cells in mice transduced with control shRNA (top) and Lmna shRNA (bottom), n ≥ 5, scale bar = 50 μm.