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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Circ Res. 2019 Sep 9;125(9):834–846. doi: 10.1161/CIRCRESAHA.119.314794

Figure 3. Co-regulation of Bmp7 and Ctgf suppresses Lmna DCM and cardiac fibrosis.

Figure 3.

(a and c) H&E (left panel) and MT (middle panel) staining of paraffin heart sections of Lmna DCM mice 4 weeks after (a) EGFP-Ctrl shRNA, Yy1-Ctrl shRNA or Yy1-Bmp7 shRNA transduction and (c) EGFP-Ctrl shRNA or Bmp7-Ctgf shRNA transduction. Quantification of myocardial fibrosis (right panel) from MT-stained sections, n ≥ 6. Scale bars: 1000 μm for complete heart images; 100 μm for enlarged images. (b) Quantitative real-time PCR analyses of Bmp7, Col1a1 and Col1a2 expression in Lmna DCM groups treated with EGFP-Ctrl shRNA, Yy1-Ctrl shRNA or Yy1-Bmp7 shRNA, n ≥ 5. (d) Quantitative real-time PCR analyses of Myh7, Nppa, Col1a1 and Col1a2 expressions in Lmna DCM groups treated with EGFP-Ctrl shRNA or Bmp7-Ctgf shRNA groups, n ≥ 6.