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. 2020 Feb 5;18(8):1810–1829. doi: 10.1111/pbi.13343

Figure 3.

Figure 3

Localization of GmSNAP18 protein at the plasma membrane in soybean in SCN‐infected root. (a, b, c) Immunostaining, (d) In situ hybridization. (a) A specific expression of the GmSNAP18 protein at the plasma membrane was detected in the SCN‐resistant Forrest using anti‐SNAP18 polyclonal antibodies after immunostaining (b) In the SCN‐susceptible Essex, absence of GmSNAP18 expression was seen in the syncytial feeding cells at 3 days after inoculation with SCN. (c) Absence of GmSNAP18 expression at the plasma membrane in negative controls. (d) Expression of GmSNAP18 in the SCN‐infected area was also detected by in situ hybridization using the specific GmSNAP18 probe in ‘Forrest’ soybean roots. The GmSNAP18 expression is induced by SCN infection. GmSNAP18 proteins hyper‐accumulated at the plasma membrane, and their expression was specific to the syncytial feeding cells.