Figure 7.

BiFC analysis of GmSNAP18, GmSHMT08 and GmPR08‐Bet VI. The coding sequences of Forrest (f) and Essex (e) wild‐type GmSHMT08 were cloned into pSAT4‐nEYFP‐C1 (E81) to generate nEYFP‐GmSHMT08 fusions. Likewise, GmSNAP18 from Forrest and Essex wild‐type and GmPR08‐Bet VI coding sequences were cloned into pSAT4‐cEYFP‐C1‐B (E82) and pG2RNAi2 to generate cEYFP‐GmSNAP18 and pG2RNAi2‐GmPR08‐Bet VI fusions. Various combinations of cEYFP and nEYFP fusions including controls (Figure S10) were co‐expressed in onion epidermal cells by particle bombardment. Interactions were stronger when the GmPR08‐Bet VI was present, and when both GmSNAP18 and GmSHMT08 were present as resistant alleles. However, weak interactions were observed when at least one of the three partners was missing or present as a susceptible allele. Bar = 200 µm.