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. 2020 Jan 30;18(8):1736–1748. doi: 10.1111/pbi.13337

Figure 2.

Figure 2

Regions in MdMYB1 promoter with different methylation levels among ‘Nagafu 2’, ‘Yanfu 3’ and ‘Yanfu 8’. (a) Methylation levels at different promoter regions of MdMYB1 in ‘Nagafu 2’, ‘Yanfu 3’ and ‘Yanfu 8’ determined by McrBC‐PCR with genomic DNA from fruit skin samples (12 DABR) as the template. Promoter of MdMYB1 was divided into four regions (MR1–MR4; location of each region is marked). Negative control template contained water instead of GTP. M and C represent McrBC‐digestion groups with or without GTP, respectively. Plasmid DNA was used as positive control. (b) Methylation levels of MR3 promoter region of MdMYB1 determined by BSP. On x‐axis, ‘All’ represents overall methylated cytosine, while CG, CHG and CHH represent three different types of cytosine methylation. (c) Average percentage of methylation at each cytosine in three cultivars. Error bars show standard deviation of data from triplicate gDNA extractions with three technical replicates, ‘*’ and ‘**’ indicate significance difference at P < 0.05 and P < 0.01, respectively.