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. 2020 Jan 30;18(8):1736–1748. doi: 10.1111/pbi.13337

Figure 4.

Figure 4

Heterologous expression of MdAGO4s or MdDRM2s rescues CHH methylation of AGO4‐binding sites in Arabidopsis ago4 and drm2 mutants. (a) Ten‐day‐old wild‐type Arabidopsis (WT), ago4 mutant, drm2 mutant and transgenic Arabidopsis (35S::AGO4‐1/2 and 35S::DRM2‐1/2). DNA methylation was analysed using three methylation‐sensitive restriction endonucleases, AluI (b), HaeIII (c) and DdeI (d). Genomic DNA was extracted from 2‐week‐old seedlings, and digested genomic DNAs were amplified by PCR. Sequences lacking AluI sites (IGN5), HaeIII (AT5G27860) and DdeI (AT2G36490) were used as loading controls. Sequence and size of Arabidopsis genes are listed in Table S2. 1–7 indicate 35S::AGO4‐1/ago4, 35S::AGO4‐2/ago4, ago4, WT, drm2, 35S::DRM2‐1/drm2 and 35S::DRM2‐2/drm2, respectively.