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. 2020 Jan 24;18(8):1683–1696. doi: 10.1111/pbi.13332

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© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Functional assessment of HvAKT2 and HvHAK1 via BSMV‐VIGS. (a) Phenotype after silencing of HvAKT2 and HvHAK1 in wild barley XZ5 via BSMV‐VIGS. Control and drought correspond to basic nutrition solution (BNS) and BNS + 20% PEG, respectively. (b) qRT‐PCR analysis of HvHAK1 and HvAKT2 in leaves of XZ5. (c) Biomass after silencing of HvAKT2 and HvHAK1 in XZ5 via BSMV‐VIGS. (d) K concentration in leaves of plants after silencing of HvAKT2 and HvHAK1 in XZ5 via BSMV‐VIGS. Inoculated seedlings were grown in BNS for 10 days, followed by PEG‐induced drought for 5 days. Data are mean ± SD (n = 6), and different letters indicate significant differences (P < 0.05).