Fig. 6.

Lymphocyte T proliferation from IBDV immune chickens. Spleen mononuclear cells isolated from 16-weeks old chickens inoculated on day 0 with vectorized vaccine [FARMUNE® (HVT-IBDV-ILTV)], were stimulated in duplicate with 1 μg/ml ConA or 10⁷ copies/ml of inactivated IBDV (infectious bursal disease virus - recall antigen). As basal control, cells were cultured only with medium. (a) Flow cytometry analysis protocol used to evaluate the proliferation of CD8⁺ and CD4⁺ cells. (b) Quantitative data showing the proliferation of CD4⁺ or CD8⁺ T cells. Each dot represents an animal. All values shown are percentage of CD4⁺ or CD8⁺ cells. The results are expressed as the mean ± standard deviation of one experiment. Significant differences are indicated by *(p = 0.0286). Per sample, 50,000 events were acquired using a FACSCanto II flow cytometer