Upregulation of the expression of long noncoding RNA OSER1-AS1 in NSCLC is associated with poor prognosis. (A) Evaluation of OSER1-AS1 expression in 53 pairs of NSCLC tissues and adjacent normal tissues by RT-qPCR. **P<0.01, compared with the adjacent normal tissues. (B) Clarification of the OSER1-AS1 expression status in five human NSCLC cell lines (H522, H460, H1703, A549, and SK-MES-1) using RT-qPCR. The human nontumorigenic bronchial epithelial cell line BEAS-2B served as the control. **P<0.01, compared with the BEAS-2B cells. (C) Comparison of the overall survival of patients with NSCLC in the high- and low-OSER1-AS1 expression groups (P=0.0253). OSER1-AS1, RNA OSER1 antisense RNA 1; NSCLC, non-small cell lung cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.