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MCF-7 and MDA-MB-231 cell lines with stable SBEM knockdown or overexpression are successfully established. SBEM protein expression was significantly decreased after sh-SBEM was transfected), and was significantly increased after the overexpression plasmid was transfected in (A) MCF-7 and (B) MDA-MB-231 cells. *P<0.05 and **P<0.01, compared with the scramble or vector group. SBEM, small breast epithelial mucin.
