Expression of TSPYL5 was downregulated in CRC tissues. (A) TSPYL5 expression levels in COAD (colon adenocarcinoma) T, tumor [n=275], N, normal [n=349] and READ (rectum adenocarcinoma) T [n=92], N [n=318] were investigated via the GEPIA database. (B) The levels of TSPYL5 mRNA expression in 30 paired tumor tissues and para-carcinoma tissues were investigated by quantitative real-time PCR analysis. (C) The levels of TSPYL5 protein expression in 8 paired tumor tissues (T1-T8) and para-carcinoma tissues (P1-P8) were investigated by western blotting. (D) Quantitative analysis of TSPYL5 protein levels in 8 paired tumor tissues and para-carcinoma tissues. *P<0.05, **P<0.01. P, para-carcinoma tissues; T, CRC tumor tissues. CRC, colorectal cancer; TSPYL5, testis-specific protein Y-encoded-like 5; GEPIA, Gene Expression Profiling Interactive Analysis. T, Tumor tissues; N, non-tumor tissues; n, number of samples used.