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Validation of expression of TSPYL5 in CRC HCT116 and HT29 cells lines after transfection with the overexpression vector. (A) RT-qPCR was used to measure the mRNA expression of TSPYL5. (B) Western blot analysis was used to detect the expression of TSPYL5 protein. ***P<0.001, compared to the negative control (NC) group. CRC, colorectal cancer; TSPYL5, testis-specific protein Y-encoded-like 5.
