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. 2020 Jun 9;44(2):449–456. doi: 10.3892/or.2020.7639

Figure 6.

Figure 6.

Effects of TSPYL5 overexpression on ERS and its associated proteins. HCT116 and HT29 cells were transfected with pcDNA3.1-TSPYL5 or empty pcDNA3.1, and subsequently divided into TSPYL5 and negative control (NC) groups, respectively. (A) Western blot analysis of the protein levels of caspase-1, caspase-3, bcl-2-like protein 4 (Bax), activating transcription factor 4 (ATF4) and CCAAT-enhancer-binding protein homologous protein (CHOP) in the HT29 and HCT116 cells in the TSPYL5 overexpression and NC groups. (B) Quantification of the levels of caspase-1, caspase-3, Bax, ATF4, and CHOP protein expression as determined by western blot analysis. *P<0.05, **P<0.01 and ***P<0.001 vs. the NC group. CRC, colorectal cancer; TSPYL5, testis-specific protein Y-encoded-like 5.