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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Transgenic Res. 2017 Jun 12;26(4):477–489. doi: 10.1007/s11248-017-0025-2

Figure 1. Isolating Long Evans rat laminin-binding spermatogonia.

Figure 1.

a) Matrix selection method used to isolate Long Evans rat spermatogonial stem cells in culture. Germ cells were harvested from Somatic Cells bound to plastic by gentle pipetting, and plated onto collagen-I for ~4 hr. Collagen Non-Binding germ cells were harvested and incubated on laminin ~40 min to collect laminin Non-Binding (LNB) and Laminin-Binding (LB) spermatogenic cell fractions.

b) Primary cultures of Long Evans rat testis cells (Germ Cells + Somatic Cells) isolated from 23 day old rats as described in panel “a”. Yellow and cyan arrows point to Spermatogenic-like (Germ Cells) and somatic-like testis cells (Somatic Cells), respectively. Scale, 40 μm