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. Author manuscript; available in PMC: 2020 Jul 6.
Published in final edited form as: Cell Rep. 2018 Nov 13;25(7):1829–1840.e6. doi: 10.1016/j.celrep.2018.10.055

Figure 2. Endogenous ERS-Containing Proteins Are Secreted in Response to Calcium Dysregulation.

Figure 2.

(A) Mass spectrometry identified 1,805 proteins from SH-SY5Y cell culture media following treatment with 200 nM Tg for 8 hr (circles) or with 50 μM dantrolene pre-treatment for 30 min (triangles) (n = 3). Peptides of putative endogenous ERS (+) proteins are identified in red. Green arrows indicate proteins known to interact with ERS (+) proteins.

(B) Fold change in peptide fragments identified in Tg versus vehicle treated media for detectable ERS (+) proteins (n = 3; unpaired two-tailed t tests; *p < 0.05, **p < 0.01, ***p < 0.001 for Tg versus vehicle; #p < 0.05, ##p < 0.01 for Tg versus Tg plus dantrolene).

(C) western blots of cell culture media and lysates for a selected set of putative endogenous ERS (+) proteins following treatment with 200 nM Tg or vehicle for 8 hr.

(D) Densitometry analysis of western blots (n = 3; unpaired two-tailed t test; *p < 0.05, **p < 0.01, ***p < 0.001).

See also Figure S3.