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Effect of reuterin on thiol-mediated reduction of Ellman's reagent. Increasing amounts of iodoacetamide (▴), reuterin (•) or control supernatant (▪) (derived from L. reuteri PRB94 cells, which have deletions of the pduC gene) were incubated in phosphate buffer with glutathione for 1 h, after which time Ellman's reagent was added and the amount of TNB produced was determined. There was no detectable TNB production when glutathione was omitted.
