Table 2.
The most highly overexpressed genes in E. coli cells treated with sublethal concentrations of reuterin
| Gene | Fold induction* | Function |
|---|---|---|
| bhs† | 35.7 | Protein involved in stress resistance and biofilm formation |
| marA | 23.3 | Transcriptional activator of multiple antibiotic resistance |
| yqhD | 21.9 | Putative oxidoreductase |
| nemR | 20.5 | Putative transcriptional regulator inactivated by Cys-modification reagents |
| marR | 15.5 | Repressor of multiple antibiotic resistance |
| grxA† | 14.3 | Glutaredoxin 1 |
| ybiJ | 13.6 | Putative periplasmic protein of unknown function |
| nemA | 13.1 | N-Ethylmaleimide reductase |
| yqhC | 10.3 | Putative ARAC-type regulatory protein |
| gloA | 8.6 | Lactoylglutathione lyase |
| ahpF† | 8.5 | Alkyl hydroperoxide reductase, F52a subunit |
| yhcN | 7.4 | Putative periplasmic protein of unknown function |
| mdaB | 5.2 | Modulator of drug activity B |
| ahpC† | 5.0 | Alkyl hydroperoxide reductase, C22 subunit |
| yjbM | 4.8 | Function unknown |
| yjgI | 4.6 | Putative oxidoreductase |
| emrR | 4.6 | Regulator of plasmid mcrB operon (microcin B17 synthesis) |
| katG† | 4.6 | Catalase hydrogen peroxidase I |
| frmR | 4.5 | Negative regulator of frmRAB operon; expression is induced by formaldehyde |
| marB | 4.5 | Multiple antibiotic resistance protein |
| adhC | 4.1 | Glutathione-dependent alcohol dehydrogenase |
| cysD | 4.1 | Sulfate adenylyltransferase, subunit 2 |
| ibpB | 4.1 | Heat-shock protein |
| rnt | 4.0 | RNase T |
| nfnB | 3.9 | Oxygen-insensitive NAD(P)H nitroreductase |
| dkgA | 3.9 | 2,5-Diketo-d-gluconate reductase A |
| yafB | 3.8 | Putative aldose reductase |
| yegQ | 3.6 | ORF, hypothetical protein |
| iscR† | 3.6 | Transcriptional repressor for isc operon |
| trxC† | 3.5 | Thioredoxin 2 |
| iscS† | 3.4 | Putative aminotransferase |
| adiY | 3.3 | Putative ARAC-type regulatory protein |
| ibpA† | 3.3 | Heat-shock protein |
*Increased level of expression in reuterin-treated cells versus untreated cells.
†Genes overexpressed in response to hydrogen peroxide (Zheng et al., 2001).