Fig. 1.

Cystatin C and kininogens in human plasma interact with immobilized SIC. SIC (circles) or the albumin binding protein PAB of F. magnus (triangles) were immobilized on microtitre plates and incubated with plasma in dilution series starting at 1 : 20. When binding of cystatin C was investigated, the plasma was supplemented with 10 μg cystatin C ml^–1 prior to dilution. After washing, bound cystatin C was detected with specific cystatin C antibodies (closed symbols), and kininogens with antibodies recognizing both HK and LK (open symbols). Bound antibodies were visualized after incubation with peroxidase-labelled secondary antibodies. Results from one of three representative experiments are shown.