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SIC binds to domains D3 and D5 of HK. Microtitre plates were coated with dilution series of SIC and incubated with domain D3 (•) or domain D5 (○) of HK (both at 2 μg ml−1). After washing, bound proteins were detected with a polyclonal antiserum against HK. Bound antibodies were detected with peroxidase-labelled secondary antibodies. Results from one of at least three representative experiments are shown.
