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SIC blocks BK generation. Twenty-five microlitres of human plasma was incubated with 25 μl SIC of various concentrations for 30 min. After the addition of the aPTT reagent kaolin (50 μl), the amount of BK in 2 μl of the reaction mixtures was determined by a competitive ELISA. Plasma not incubated with SIC or kaolin (‘no aPTT reagent’) and plasma activated by kaolin in the absence of SIC (‘plus aPTT reagent’) were negative and positive controls, respectively. Bars, sem.
