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. 2014 Apr;160(Pt 4):671–681. doi: 10.1099/mic.0.073551-0

Table 1.

Bacterial strains and plasmids

Strain or plasmid Relevant characteristics Reference
Strains
Lactobacillus reuteri
6475 ATCC PTA 6475 (earlier designated MM4-1A); WT, host strain; human breast milk (a kind gift from BioGaia AB, Stockholm, Sweden) Oh et al. (2010)
6475 cmbA Derivative of 6475; nonsense mutation in cmbA (hmpref0536_10633) encoding a hypothetical LPxTG motif containing protein* This work
6475 10146 Derivative of 6475; nonsense mutation in hmpref0536_10146 encoding a hypothetical LPxTG motif containing protein* This work
6475 11993 Derivative of 6475; nonsense mutation in hmpref0536_11993 encoding a putative C-terminal membrane-anchored cell surface protein* This work
6475 10802 Derivative of 6475; nonsense mutation in hmpref0536_10802 encoding a LPxTG motif containing amidase* This work
6475 10154 Derivative of 6475; nonsense mutation in hmpref0536_10154 encoding a LPxTG motif containing Ser/Thr protein phosphatase* This work
6475 srtA Derivative of 6475; nonsense mutation in srtA (hmpref0536_10255) encoding sortase A* This work
6475(pSIPΔ) Derivative of 6475 containing pSIPΔ This work
6475(pSIP-cmbA) Derivative of 6475 containing pSIP-cmbA This work
6475 cmbA(pSIPΔ) Derivative of 6475 cmbA containing pSIPΔ This work
6475 cmbA(pSIP-cmbA) Derivative of 6475 cmbA containing pSIP-cmbA This work
6475 srtA(pSIPΔ) Derivative of 6475 srtA containing pSIPΔ This work
6475 srtA(pSIP-srtA) Derivative of 6475 srtA containing pSIP-srtA This work
Lactococcus lactis
MG1363 Intermediate cloning host Gasson (1983)
Plasmids
pSIP411 Emr; SppIP-based expression vector with PsppQ : : gusA Sørvig et al. (2005)
pSIPΔ Emr; pSIP411 derivative without gusA This work
pSIP-cmbA Emr; pSIP411 derivative containing cmbA under control of PsppQ This work
pSIP-srtA Emr; pSIP411 derivative containing srtA under control of PsppQ This work
*

Details given in Table S1.