Table 1.
Bacterial strains and plasmids
Strain or plasmid | Relevant characteristics | Reference |
Strains | ||
Lactobacillus reuteri | ||
6475 | ATCC PTA 6475 (earlier designated MM4-1A); WT, host strain; human breast milk (a kind gift from BioGaia AB, Stockholm, Sweden) | Oh et al. (2010) |
6475 cmbA− | Derivative of 6475; nonsense mutation in cmbA (hmpref0536_10633) encoding a hypothetical LPxTG motif containing protein* | This work |
6475 10146 − | Derivative of 6475; nonsense mutation in hmpref0536_10146 encoding a hypothetical LPxTG motif containing protein* | This work |
6475 11993 − | Derivative of 6475; nonsense mutation in hmpref0536_11993 encoding a putative C-terminal membrane-anchored cell surface protein* | This work |
6475 10802 − | Derivative of 6475; nonsense mutation in hmpref0536_10802 encoding a LPxTG motif containing amidase* | This work |
6475 10154 − | Derivative of 6475; nonsense mutation in hmpref0536_10154 encoding a LPxTG motif containing Ser/Thr protein phosphatase* | This work |
6475 srtA− | Derivative of 6475; nonsense mutation in srtA (hmpref0536_10255) encoding sortase A* | This work |
6475(pSIPΔ) | Derivative of 6475 containing pSIPΔ | This work |
6475(pSIP-cmbA) | Derivative of 6475 containing pSIP-cmbA | This work |
6475 cmbA−(pSIPΔ) | Derivative of 6475 cmbA− containing pSIPΔ | This work |
6475 cmbA−(pSIP-cmbA) | Derivative of 6475 cmbA− containing pSIP-cmbA | This work |
6475 srtA−(pSIPΔ) | Derivative of 6475 srtA− containing pSIPΔ | This work |
6475 srtA−(pSIP-srtA) | Derivative of 6475 srtA− containing pSIP-srtA | This work |
Lactococcus lactis | ||
MG1363 | Intermediate cloning host | Gasson (1983) |
Plasmids | ||
pSIP411 | Emr; SppIP-based expression vector with PsppQ : : gusA | Sørvig et al. (2005) |
pSIPΔ | Emr; pSIP411 derivative without gusA | This work |
pSIP-cmbA | Emr; pSIP411 derivative containing cmbA under control of PsppQ | This work |
pSIP-srtA | Emr; pSIP411 derivative containing srtA under control of PsppQ | This work |
Details given in Table S1.