Table 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristics	Reference
Strains
Lactobacillus reuteri
6475	ATCC PTA 6475 (earlier designated MM4-1A); WT, host strain; human breast milk (a kind gift from BioGaia AB, Stockholm, Sweden)	Oh et al. (2010)
6475 cmbA−	Derivative of 6475; nonsense mutation in cmbA (hmpref0536_10633) encoding a hypothetical LPxTG motif containing protein*	This work
6475 10146 −	Derivative of 6475; nonsense mutation in hmpref0536_10146 encoding a hypothetical LPxTG motif containing protein*	This work
6475 11993 −	Derivative of 6475; nonsense mutation in hmpref0536_11993 encoding a putative C-terminal membrane-anchored cell surface protein*	This work
6475 10802 −	Derivative of 6475; nonsense mutation in hmpref0536_10802 encoding a LPxTG motif containing amidase*	This work
6475 10154 −	Derivative of 6475; nonsense mutation in hmpref0536_10154 encoding a LPxTG motif containing Ser/Thr protein phosphatase*	This work
6475 srtA−	Derivative of 6475; nonsense mutation in srtA (hmpref0536_10255) encoding sortase A*	This work
6475(pSIPΔ)	Derivative of 6475 containing pSIPΔ	This work
6475(pSIP-cmbA)	Derivative of 6475 containing pSIP-cmbA	This work
6475 cmbA−(pSIPΔ)	Derivative of 6475 cmbA− containing pSIPΔ	This work
6475 cmbA−(pSIP-cmbA)	Derivative of 6475 cmbA− containing pSIP-cmbA	This work
6475 srtA−(pSIPΔ)	Derivative of 6475 srtA− containing pSIPΔ	This work
6475 srtA−(pSIP-srtA)	Derivative of 6475 srtA− containing pSIP-srtA	This work
Lactococcus lactis
MG1363	Intermediate cloning host	Gasson (1983)
Plasmids
pSIP411	Emr; SppIP-based expression vector with PsppQ : : gusA	Sørvig et al. (2005)
pSIPΔ	Emr; pSIP411 derivative without gusA	This work
pSIP-cmbA	Emr; pSIP411 derivative containing cmbA under control of PsppQ	This work
pSIP-srtA	Emr; pSIP411 derivative containing srtA under control of PsppQ	This work

^*Details given in Table S1.