FIGURE 6.

Adipose tissue-specific deletion of DRP1 causes decreased lipolysis and unhealthy adipocytes. A, Western blotting analysis of the protein levels for lipolysis-related proteins, including pHSL (Ser660), HSL, ATGL, CGI58, and PLIN1 in the sWAT from Adipo-Drp1^flx/flx mice and their littermate controls after cold exposure. β-Actin is used as the loading control. B, Quantification of relative band intensity (normalized to β-Actin) in (A) (n = 6 per group, the sample loaded in each lane contains pooled samples from two mice, representative of three trials. The data are represented as mean ± SEM, Student’s t test, *P < .05, **P < .01). C, Western blotting (WB) analysis of the protein levels for lipolysis-related proteins, including pHSL (Ser660), HSL, ATGL, CGI58, and PLIN1 in the BAT from Adipo-Drp1^flx/flx mice and their littermate controls after cold exposure. β-Actin is used as the loading control. D, Quantification of relative band intensity (normalized to β-Actin) in (C) (n = 6 per group, representative of three trials. The data are represented as mean ± SEM, Student’s t test, *P < .05). E, Glycerol levels in the serum of Adipo-Drp1^flx/flx mice and their littermate controls after cold exposure (n = 6 per group, representative of three trials, data are represented as mean ± SEM, Student’s t test, ***P < .001). F, Glycerol levels in the sWAT and BAT from Adipo-Drp1^flx/flx mice and their littermate controls after cold exposure (n = 6 per group, representative of three trials, data are represented as mean ± SEM., Student’s t test, **P < .01, ***P < .001). G, Free fatty acid (FFA) levels in the sWAT and BAT from Adipo-Drp1^flx/flx mice and their littermate controls after cold exposure (n = 6 per group, representative of three trials, data are represented as mean ± SEM, Student’s t test, **P < .01)