Figure 2.
Expression of defence marker genes. Relative expression of defence marker genes in tomato plants infected with Meloidogyne incognita (Mi), infested with Spodoptera exigua (Se) or both (MiSe). Con = plants without herbivory. Expression values are normalized over the expression of the housekeeping gene (SIEF X14449) encoding for tomato elongation factor-1α. Box plots are mean (±SEM) expression values of Lipoxygenase D (LoxD); Leucine aminopeptidase A (LapA); abscisic acid-responsive Le4 (Le4); Basic-β-1-glucanase (GluB) per treatment (n = 5) measured at the nematodes’ invasion (A–D), galling (E–H) and reproduction (I–L) stages, respectively. Different lower-case letters above the box plots indicate significant differences in mean expression among treatments, determined via multiple comparisons Tukey’s HSD test after ANOVA at P ≤ 0.05.