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. 2020 Jun 24;12(4):plaa029. doi: 10.1093/aobpla/plaa029

Figure 3.

Figure 3.

Expression of steroidal glycoalkaloid metabolism genes. Relative expression of steroidal glycoalkaloid metabolism genes in tomato plants infected with Meloidogyne incognita (Mi), infested with Spodoptera exigua (Se) or both (MiSe). Con = plants without herbivory. Expression values are normalized over the expression of the housekeeping gene (SIEF X14449) encoding for tomato elongation factor-1α. Box plots are mean (±SEM) expression values of jasmonate-responsive ETHYLENE RESPONSE FACTOR 4 (JRE4; A, C, E); and glycoalkaloid metabolism 1 (GAME1; B, D, F) per treatment (n = 5) measured at the nematodes’ invasion (A and B), galling (C and D) and reproduction (E and F) stages, respectively. Different lower-case letters above the box plots indicate significant differences in mean expression among treatments, determined via multiple comparisons Tukey’s HSD test after ANOVA at P ≤ 0.05.