Figure 5.
Suppression of early neutrophil influx into the airways does not require plasminogen activator protease (Pla) proteolytic activity. A, Flow cytometry plots representing neutrophil abundance in the bronchoalveolar lavage fluid (BALF) of a single mouse infected intranasally with 5 × 104 colony-forming units (CFUs) of CO92, CO92 Δpla, CO92 Δpla + pla WT, CO92 Δpla + plaS99A, or CO92 Δpla + plaD206A at 4 and 12 hours after infection. Gate contains F4/80−CD11bhighLy-6G+ cells (neutrophils), and values show the percentage of the parent F4/80− population. Abbreviations: Cy7, cyanine 7; PE, phycoerythrin. B, C, Quantification of the abundance of neutrophils in the BALF of mice at 4 hours (B) and 12 hours (C) after infection, as described in A. Plots represent the number of neutrophils found in every 1000 live cells in the BALF. All experiments were performed 3 times; error bars represent standard deviations (n = 5). *P < .01; †P < .001 (2-way analysis of variance).