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. 2020 Jul 6;20:196. doi: 10.1186/s12866-020-01882-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Hemocytes from ALA-PDT treated larvae do not contribute to the killing of S. aureus and C. albicans in vitro.a The disk diffusion assay against S. aureus and C. albicans. The larval hemolymph (containing hemocytes) was collected after ALA-mediated PDT treatment. PBS, negative control was the blank paper disk impregnated with PBS. GM or ITZ, positive control was the blank paper disk impregnated with gentamicin (GM, 1 mg/mL) or itraconazole (ITZ, 16 mg/L). b Kinetics of bacterial and fungal killing by insect hemocytes. Five larvae were treated with ALA-PDT. PBS was used as control. The hemocytes were collected and pooled. S. aureus or C. albicans cells were exposed to hemocytes at the indicated timepoints. The data are from a representative experiment performed in triplicate