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[Preprint]. 2020 Aug 31:rs.3.rs-30934. [Version 3] doi: 10.21203/rs.3.rs-30934/v3

Figure 1.

Figure 1

Cleavage of ISG15 C-terminal 8 amino acids by SARS-CoV-2 PLpro purified from E. coli. ISG15 was incubated with SARS-CoV-2 PLpro (lanes 3 to 6). SARS-CoV-2 PLpro was present at 4 nM, ISG15 was present at 10 μM. For lane 4 to 6, famotidine was present at 100 μM, 10 μM and 1 μM respectively. Control was without enzyme (lane 2). Proteins were resolved by 15% SDS-PAGE and revealed by Coomassie blue staining. The molecular weights of the marker proteins are indicated on the left of the gel.