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. 2020 Jun 26;11:1445. doi: 10.3389/fmicb.2020.01445

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Copyright © 2020 de Alvarenga, Hess and Hagemann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verification of acnSP expression. A FLAG-tagged version of acnSP was expressed in strain pVZ322_AcnSP from its native promoter. 10 μg of protein were applied onto the SDS gel and FLAG-tagged proteins were detected with a commercially available antiserum. A specific signal was observed in the extracts of strain pVZ322_AcnSP but not in WT. Protein extracts of strain pVZ322-petE:hliR1 accumulating a FLAG-tagged version of the small protein HliR1 (Baumgartner et al., 2016) were included as positive control (M-protein size marker Mark12^TM Unstained Standard Ladder, Thermo Fisher Scientific, *marks the weak signal around 10 kDa recognized by the FLAG-tag antibody).