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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: Nature. 2020 Jan 8;580(7801):113–118. doi: 10.1038/s41586-019-1885-9

Figure 1. Recapitulation of the mouse and human segmentation clocks in vitro by differentiation of pluripotent stem cells towards PSM fate.

Figure 1.

a, Immunofluorescence for stage-specific markers (left) and images of the mESC pMsgn1-Venus/hiPSC MSGN1-Venus reporters (right) in differentiating mouse ESCs and human iPSCs. Scale bar = 100μm. n=7 independent experiments. b, Normalized Hes7-Achilles intensity profiles for three mESC-derived PSM cells imaged in CLFBR medium. n=17 independent experiments c, Period of Hes7-Achilles/HES7-Achilles oscillations in mouse ESC-derived PSM and human iPSC-derived PSM cells cultured in CLFBR medium. Mean ±SD. n=25 d, Heatmap of Hes7-Achilles intensity over time in mESC-derived PSM cells in CLFBR medium. Each row represents one cell. n=15 e, Normalized HES7-Achilles intensity profiles for three human iPSC-derived PSM cells imaged in CLFBR medium. n= 23 independent experiments f, Heatmap of HES7-Achilles intensity over time in human iPSC-derived PSM cells in CLFBR medium. Each row represents one cell. n=15