FIG. 6.

BMMSCs reduced damage and apoptosis of IAR20 cells after oxidative stress. (A) Morphology of IAR20 cells after stimulation with different concentrations of H₂O₂: as the concentration of H₂O₂ increased, cell deformation, shedding, and necrosis occurred, and cell damage gradually increased. (B) Evaluation of oxidative stress injury and cell viability of IAR20 cells under different H₂O₂ concentrations. As the H₂O₂ concentration increased, cell damage increased, oxidative stress injury gradually increased, and CCK-8 detection showed decreased cell activity. (C) Morphology of cocultured IAR20 cells (scale bar = 50 μm, n = 3). (D) ALT and AST levels in of cocultured IAR20 cells (ALT, I group: 1.17 ± 5.73 U/L, IH group: 22.06 ± 0.63 U/L, IH B group: 37.38 ± 1.09 U/L; AST, I group: 2.97 ± 0.38 U/L, IH group: 10.33 ± 0.76 U/L, IH B group: 8.57 ± 0.70 U/L, n = 3). (E) Apoptosis of cocultured IAR20 cells (I group: 5.98% ± 0.14%, IH group: 21.85% ± 2.88%, IH B group: 9.76% ± 0.82%, n = 3). ^$P < 0.05 versus IH group.; I, IAR20; IH, IAR20 (H₂O₂); IH B, IAR20 (H₂O₂) cocultured with BMMSCs.