Fig. 1.

Structural characterization of TGC161. (A) Chemical structure of TGC161. (B) Cytotoxicity of TGC161 in 293 T cells. 293 T cells were cultured with TGC161 at indicated concentrations for 48 h. Cell viabilities were measured by resazurin assay (n = 4). (C) Gel chromatography analysis of TGC161. Analysis was established by a TSK-Gel GMPW column on an Ultimate 3000 high performance liquid chromatographic instrument. (D) Monosaccharide composition of TGC161. (E) The IR spectrum of TGC161. The FT-IR spectrum was recorded in the region between 400 and 4000 cm⁻¹ with a KBr pellet. (F) The HSQC spectrum of TGC161. The spectrum of NMR analysis was recorded at room temperature in an Agilent DD2 superconducting NMR spectrometer, operating at 500 MHz.