Figure 2.

Effects of TF3 on RANKL-induced osteoclastogenesis and F-actin ring formation in vitro. (A) BMMs were treated with M-CSF and RANKL containing various concentrations of TF3 (0, 1, 10 μM) for 5 days, and TRAP staining was then performed. (B) TRAP⁺ multinucleated cells with more than three nuclei were counted as osteoclasts. (C) Representative images showing the F-actin formation in osteoclasts treated with TF3. n = 3, ^** P < 0.01 compared with C group. ^## P < 0.01 compared with L group. Scale bar = 100 µm.