Figure 1. 15-Deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) induced apoptosis in MCF10A-ras cells.

(A, B) MCF10A-ras cells were treated with 15d-PGJ₂ (5 and 10 μM) for 24 hours. The cell viability was determined by the conventional MTT reduction assay. Bars represent mean ± SE of three experiments. A significant difference in the relative viability between treated cells and the solvent control is indicated with P < 0.01. (B) The proteolytic cleavage of PARP and actin were examined by Western blot analysis. (C) MCF10A-ras cells were treated with 15d-PGJ₂ (10 μM) for 24 hours in the presence or absence of an pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (ZVAD-FMK). Expression of cleaved PARP and caspase-3 was detected by Western blot analysis.