Figure 3. 15-Deoxy-Δ^12,14-prostaglandin J₂ (15d-PGJ₂) suppresses the NF-κB DNA binding activity.

(A) MCF10A-ras cells were treated with 15d-PGJ₂ (10 μM) for indicated time, and the nuclear protein was isolated. Nuclear extracts from MCF10A-ras cells were incubated with [γ-³²P]-labeled oligonucleotides harboring the NF-κB-consensus sequence. (B) MCF10A-ras cells were treated with 15d-PGJ₂ in the absence or presence of N-acetyl-L-cysteine (NAC) (5 mM) for 12 hours and immunocytochemical analysis was performed by using an antibody for phospho-p65. Propidium iodide (PI) was used to stain the nuclear of MCF10A-ras cells. The stained cells were analyzed under a confocal microscope.