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. 2020 Jun 30;25(2):79–86. doi: 10.15430/JCP.2020.25.2.79

Figure 1. LSD1 inhibitor SP2509 induces apoptosis.

Figure 1

(A) Caki cells were treated with SP2509 (0.5-2 μM) for 24 hours. The cell morphology and nuclear condensation were examined by using an interference light microscope. White arrows showed nuclear chromatin condensation. (B) The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. (C) The fragmentation of the nuclei was determined by using a DNA fragmentation assay kit. (D) Caspase activity was determined by using the caspase DEVDase assay kit. (E) Caki cells were treated with SP2509 (2 μM) in the presence or absence of a pan-caspase inhibitor, z-VAD-fmk (20 μM) for 24 hours. The sub-G1 population and protein expression were analyzed by flow cytometry and Western blotting. The values in graphs represent the mean ± SD of three independent samples. DAPI, 4’, 6’-diamidino-2-phenylindole. aP < 0.01 compared to the control. bP < 0.01 compared to the treatment of SP2509.