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. 2020 Jun 30;25(2):79–86. doi: 10.15430/JCP.2020.25.2.79

Figure 3. SP2509-induced Bcl-2 downregulation contributes to induction of apoptosis.

Figure 3

(A) Vector cells (Caki/Vec) and Bcl-2 overexpressing cells (Caki/Bcl-2) were treated with SP2509 (1-2 μM) for 24 hours, the protein expression and sub-G1 were examined by Western blotting and flow cytometry. (B) Caki cells were transiently transfected with a plasmid harboring the luciferase under the control of the Bcl-2/-751 and Bcl-2/-1281 promoter and then treated with 2 μM SP2509 for 24 hours. The luciferase activity was analyzed. The graphs represent the mean ± SD from independent experiments. aP < 0.01 compared with the control in Caki/Vector. bP < 0.01 compared with the control.