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. 2020 Jun 30;25(2):79–86. doi: 10.15430/JCP.2020.25.2.79

Figure 4. LSD1 inhibitor SP2509 downregulates Mcl-1 expression in Caki cells.

Figure 4

(A, B) Vector cells (Caki/Vec) and Mcl-1-overexpressing cells (Caki/Mcl-1) were treated with SP2509 (2 μM) for 24 hours. (B) Caki cells were pretreated with MG132 (1 μM) and lactacystin (2.5 μM) for 30 minutes, then treated with SP2509 (2 μM) for 24 hours. (C) Caki cells were treated with or without SP2509 (2 μM) in the presence of cycloheximide (CHX) (20 μg/mL) for the indicated time periods. (D) Caki cells were treated with SP2509 (0.5-2 μM). The protein expression levels were examined by Western blotting. (A) The graph represents the mean ± SD from independent experiments. aP < 0.01 compared with the SP2509-treated Caki/Vector.