Fig. 3.

Cryo-EM map and model of prefusion CDV solF trimers. (A) Three different views of the prefusion CDV solF protein model and the cryo-EM map are shown. The three protomers of the model are differently colored (yellow, red and green) and displayed as a ribbon model. The cryo-EM map is visualized as a transparent volume. In the side view, the stalk region is indicated by an asterisk and the densities of the three N-linked glycosylation sites (N141, N173 and N179) are marked with arrowheads. In the bottom view, the locations of the three binding sites with the densities for the inhibitor 3G are highlighted with black circles. For more clarity, the views were slabbed. (B) The full CDV solF model is shown and viewed from the side. The trimeric nature of CDV F is indicated by three different colors for the three protomers (yellow, red and green). (C) On the left side, all the three protomers of CDV F are shown and colored according to the different structural domains as defined for the prefusion PIV5 F structure (Yin et al., 2006). On the right side, only one protomer is shown with the same coloring: HRB, cyan; Fusion Peptide (FP), black; DI, magenta; DII, orange; DIII, blue.