Table 1.
Data collection and model validation statistics.
| Data Collection | |
| Microscope | FEI Polara |
| Voltage (kV) | 300 |
| Camera | Gatan K2 Summit |
| Defocus range (µm) | 0.9 to 3.4 |
| No. of movies | 1,604 |
| Frames per movie | 48 |
| Total dose per movie (e−/Å2) | 72.8 |
| Pixel size (Å) | 0.5105 |
| Reconstruction | |
| Software | RELION-3 |
| Symmetry | C3 |
| Particles refined | 115,248 |
| Map Resolution (Å)* | 4.3 |
| Map sharpening B-factor (Å2) | −206 |
| Refinement and Validation | |
| Software | Phenix 1.16–3546 |
| Model composition | |
| Non-hydrogen atoms | 9,834 |
| Protein residues | 1,293 |
| MolProbity Score | 1.88 |
| All-atom clashscore | 10.97 |
| Rotamers | |
| Favored (%) | 98.1 |
| Outliers (%) | 0 |
| Ramachandran | |
| Favored (%) | 95.3 |
| Outliers (%) | 0 |
| RMS deviations | |
| Bond length (Å) | 1.37 |
| Bond angles (°) | 0.01 |
| Mean B-factors | |
| Protein (Å2) | 87.7 |
| CaBLAM outliers (%) | 0.9 |
| Cα Geometry outliers (%) | 0 |
| Cβ Deviations > 0.25 Å (%) | 0 |
*Resolution was determined by FSC between two half-maps using 0.143 as a cutoff.