Fig. 9.

The closed structure of rTrF colored at the patches interacting strongly with buffer components based on the $P$($I_{\mathit{score}}$) calculated at pH 5.0. A: arginine, B: histidine, C: NaCl, and D: acetate; and at pH 6.5 for E: phosphate, and F: histidine. The C-lobe is shown on the left and the N-lobe on the right. Single letter code for aminoacids is used. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)