Table 1.
Summary of the stability studies from nanoDSF (T½) and ICD (c½) and structural studies from MD (main interacting patches) and SAXS (volume fractions).
| Stability studies |
Structural studies |
||||||
|---|---|---|---|---|---|---|---|
| MD (interacting patches) |
SAXS (volume fractions) |
||||||
| T½ (°C) | c½ (M) | N-lobe | C-lobe | Partially open | Closed | Dimer + aggregates | |
| Histidine | 62.2 | 1.2 | E89-F94 | D416-D420 | 0.5 | 0.3 | 0.2 |
| NaCl | 43.4 | 1.14 | E89-F94, D236- D240, D277- K280, K291-S298 | D416-D420 | 0.5 | 0 | 0.5 |
| Acetate | 66.3 | 1.35 | E89-T93, C227-C241 | D416-D420 | 0.4 | 0.4 | 0.2 |
| Arginine | 44.1 | 1.74 | K88-T93, D229-K233, D236-H242 | N413-Q424 | 0.6 | 0 | 0.4 |