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. 2020 Apr 28;215(3):747–766. doi: 10.1534/genetics.119.302985

Figure 8.

Figure 8

Effects of genetic deletion of PLD in SOD1G93A Mice. Survival, weight, and behavior were measured every 10 days starting from P 100 (post natal day 100). (A) Elimination of PLD1, PLD2, or PLD1/2 combined had no effect on survival of SOD1G93A transgenic animals. (WT (n = 12), SOD1G93A (n = 16), SOD1G93A; PLD1−/− (n = 20), SOD1G93A; PLD2−/− (n = 20) and SOD1G93A; PLD1−/− and PLD2−/− (n = 20) mouse strains). (B) Starting from P 100, mice were weighed every 10 days. PLD1 and/or PLD2 elimination had no effect on the weight loss observed in the SOD1G93A animals. (C and E) Fore limb and hind limb grip strength test measured at P 120 and 130 showed significant improvement of both fore limb and hind limb strength in SOD1G93A; PLD1−/− strains, compared to SOD1G93A controls (* P < 0.05, two-way ANOVA, followed by Tukey’s multiple comparisons test). In addition, SOD1G93A; PLD2−/− mice had greater fore limb strength and SOD1G93A; PLD1 and PLD2 greater hind limb strength at P 120, compared to the SOD1G93A mice (* P < 0.05) (n = 10 in SOD1 group and n = 20 in other groups). (D and F) Percentage change in grip strength from P 100 to P 120 (P 100 value − P 120 value)/P 100 value. Consistent with data in C and E, PLD knockout had mild beneficial effects in terms of fore and hind limb strength. (G and H) Inverted grip strength of the SOD1G93A; PLD1−/− and SOD1G93A; PLD2−/− mice is significantly improved at P 120 and P 130, respectively, compared to the SOD1G93A control animals (* P < 0.05 compared to SOD1G93A group, two-way ANOVA, followed by Tukey’s multiple comparisons test) (n = 10 in SOD1G93A group and n = 20 in other groups), meanwhile the percentage change was also analyzed and showed similar results.