Figure 5.

Analysis of cis-regulatory elements of the ace-2 locus. (A) Overview of the ace-2 locus. Schematic of ace-2 reporters and their expression in hermaphrodites. The constructs are referred to the ace-2 locus to scale. (B) In the images two ace-2 reporters are shown in red and the cho-1 fosmid (otIs354) is also shown in green as a landmark. Expression of the ace-2 fosmid was characterized in Kratsios et al. (2011) and Pereira et al. (2015). Constructs prom1 and prom2 were described in Kratsios et al. (2011), but cell identification in the head and tail neurons was not performed. Within the matrix, gray color indicates expression, “X” indicates no expression, and white indicates not determined. Some representative images of two reporter constructs are shown (also containing a cho-1 fosmid landmark reporter). Usually two or three lines were scored for each reporter and > 10 animals/line. Bar, 50 μm. PAG, preanal ganglion; RVG, retrovesicular ganglion; VNC, ventral nerve cord.