Figure 1.

Strains expressing Ssa3 or Ssa4 showed reduction in v-Src toxicity. (A) S. cerevisiae strains A1–A4 were transformed with pRS316 (EV) or pRS316P_GAL1-FLAG-v-Src (FLAG-v-Src). Growth of transformants is shown on SD and SGal solid media after 3 and 5 days, respectively, at 30°. (B) Graph represents percentage numbers of colonies grown on SGal growth media. Equal numbers of cells were plated onto SD and SGal media, and percentage growth on SGal was calculated with respect to SD media. (C) Cells grown in selective liquid SD media were washed and serially diluted onto SD and SGal media. Growth is shown after 4 days of incubation at 30°. (D) First, 5–6 transformants were pooled and grown in selective liquid SD media. Cells were reinoculated at OD_600nm 0.02 into SD or SGal media. Shown is the OD_600nm of each cell culture after 72 hr of incubation at 30°. Error bar represents SD from three different biological replicates. P-values were calculated using the Student’s t-test and A2 as a control. EV, empty vector; SGal, SD media supplemented with 2% raffinose, 2% galactose and 2% dextrose respectively.