Figure 3.
A2 and A4 strains show similar abundance of major chaperones. Yeast lysate was prepared from the indicated strains overexpressing v-Src for 12 hr. (A) Equal amounts of the cellular lysate (upper panel) or purified His6-tagged Ssa Hsp70 (Lower panel) were loaded onto 10% SDS-PAGE gels and probed with anti-Hsp70 antibody. CBB was used to stain purified Hsp70s as loading control. (B) Equal amounts of cellular lysates from indicated strains were probed with antibodies against Ydj1, Sse1, Hsp90, or Hsp104. Right panel shows quantification of respective immunoblots. Error bars represent SD from three different biological replicates. A.U., arbitrary units; CBB, Coomassie Brilliant Blue