Figure 5.
The Hsp82 interaction is similar with Ssa2 and Ssa4 isoforms of Hsp70. The purified His6-Hsp82 was adsorbed onto cobalt metal affinity resin and further incubated with yeast lysate from A2 or A4 strains expressing FLAG-v-Src. The bound fractions were probed for Ssa Hsp70s, v-Src, or Hsp82 with anti-Hsp70 antibody, anti-FLAG antibody, or anti-Hsp90 antibody, respectively. Panel toward right depicts quantification of respective western blots. Error bar represents SD from three different biological replicates. P-values were calculated using the Student’s t-test and A2 as a control.