qRT-PCR of FT expression in mac3A, mac3B, and mac3A/mac3B mutants. FT expression was measured using qRT-PCR in wild-type or homozygous (A) mac3a, (B) mac3b, and (C) mac3a/mac3b mutants grown under long-day (16 hr light/8 hr dark) conditions. Quantifications are the average of three biological replicates with error bars showing SD. qRT-PCR, quantitative real-time PCR.